The metabolism of yeast nucleic acid in the rat.

نویسندگان

  • P M ROLL
  • G B BROWN
چکیده

Recent studies on the fate of labeled dietary purines in the rat have shown t,hat dietary adenine is a precursor of t,he adenine and of the guanine of tissue nucleic acids (l), but that guanine (2), hypoxanthine (3), and xanthine (3) are not utilized for nucleic acid synthesis. Similar experiments have shown that the pyrimidines, uracil, thymine (2), and cytosine (4) are not incorporated into the nucleic acids of t’he rat. However, erotic acid (4-carboxyuracil), while not a nucleic acid component, is utilized as a precursor of the uracil and cytosine of nucleic acids (5). Since none of the pyrimidines occurring in nucleic acids are utilized when they are present in the diet, it seems probable that derivatives of these compounds are first synthesized in a way which does not involve the participation of these free pyrimidines, but which may involve erotic acid (6), and that these derivatives are then incorporated into the nucleic acids. Such derivatives could be the pyrimidine nucleosides or nucleotides. Because free guanine is not a precursor of nucleic acid guanine, it has been suggested (1) that similar derivatives of the purines may be involved in the conversion of adenine to nucleic acid guanine. Up to now, no nucleic acid component more complex than the purines or pyrimidines has been studied by the kacer technique. The availability of ribonucleic acid labeled with isotopic nitrogen (7) now enables us to extend these studies to nucleic acids. The fate of exogenous nucleic acids, nucleotides, and nucleosides has been studied in the past by the method of feeding or injecting these compounds into animals in nitrogen balance and determining what changes occur in the quantities of the various constituents of the excreta. In this way it has been found in several species that, following the administration of nucleic acids, the nitrogen of the purines could be largely, but not always completely, accounted for in the extra allantoin, uric acid, or other free purines (8-ll), but that pyrimidines were absent from the urine and that their nitrogen probably had been converted to urea (12, 13). The metabolism

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 180 1  شماره 

صفحات  -

تاریخ انتشار 1949